Light and Scanning-Beam Electron Microscopy of Wall-Defective Staphylococcus aureus Induced by Lysostaphin
نویسندگان
چکیده
منابع مشابه
Direct observation of Staphylococcus aureus cell wall digestion by lysostaphin.
The advent of Staphylococcus aureus strains that are resistant to virtually all antibiotics has increased the need for new antistaphylococcal agents. An example of such a potential therapeutic is lysostaphin, an enzyme that specifically cleaves the S. aureus peptidoglycan, thereby lysing the bacteria. Here we tracked over time the structural and physical dynamics of single S. aureus cells expos...
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Staphylococcus aureus FDA 209P cells when suspended in 24% (w/v) NaCl were rendered osmotically fragile by exposure to lysostaphin for time intervals ranging from 2 to 60 min. Such cells were analyzed chemically and serologically for evidence of residual cell wall material, were plated in hypertonic sucrose medium to determine revertibility to normal, and were subjected to manometric studies to...
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Morphological and histochemical study of the abomasal epithelium in camel (Camelus dromedarius) wascarried out by light and scanning electron microscopes. The lining of the abomasum was divided into fourregions, i.e. cardiac, pseudocardiac, fundic and pyloric. Our investigation revealed that the cardiac andpseudocardiac regions occupy a wide part of the abomasum in camel and it reaches approxim...
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The kinetics of cell-wall hydrolysis of Staphylococcus aureus (strain Cowan I) by lysostaphin was studied by turbidity, quantitative determination of hexosamines, phosphorus, total proteins, protein A and by electron microscopy. In our experimental conditions, the lytic activity of lysostaphin was very fast and no characteristic cell-wall structures could be seen after five minutes. The release...
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ژورنال
عنوان ژورنال: Infection and Immunity
سال: 1971
ISSN: 0019-9567,1098-5522
DOI: 10.1128/iai.4.1.73-78.1971